LOCUS       CO897952                 470 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09e09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93838 Q93838 F59F4.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897952
VERSION     CO897952.1  GI:51237742
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 495
            Seq primer: SL1 primer
            High quality sequence stop: 464.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tacctacccg gaagcgcaaa atggcaccta agcagcggat gaacgtggcc aatcaacaat
       61 tcagcaagaa tgtggtggcc agaggaaatg tgcaaaaatc gcttaagcca caagctaacc
      121 aatacccggc cgcaccttgg ctgatcggaa ttttcgtgtt cgttgtttgc ggttccgcga
      181 tattcgaggt tatacgctat atcaaacttg gattttgaga aggaagacgc gctaccaccg
      241 gacgttccca tttccatgta attcttcatt cttgactttt cgcacactgt acttctgtta
      301 atcatcctta aaaactgtcc tttaattgtt tacgcctatt cttccatgtt catatcatcg
      361 gctacgttgt aaacttgcaa atgggacaaa tgaaaccttt acgtgtgatc ataaatgctg
      421 ttgacaattc ttattttcta aaccgaatca aattttgagc ttaanaaaaa
//