LOCUS       CO897951                 573 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09e07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS12_PIG P46405 40S RIBOSOMAL PROTEIN S12, mRNA
            sequence.
ACCESSION   CO897951
VERSION     CO897951.1  GI:51237741
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 589
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tccattgcga ggacttccgt tttccaccga ggaaggagca atttcaacaa cttcaccaga
       61 tactcataaa acaaaatggc tgagtctctt gagatgaacg aggcgctcaa acgcgttctc
      121 aaatcagcgt tgattgtgga tggcgttgca cgtggactca acgaggctgc ccgcactctg
      181 gacaaacgtg aggctcactt ctgtgtgttc gccaagagct gcgatgaggc ctcgtacgag
      241 aagctggtgc gggccctgtg caaggagcac aacatcccgg tcatcgaggt ggaggacaag
      301 gcgcagctgg gccaaatggt tggccaatgc aagtacgaca aggacggagc tgctcgcaaa
      361 atcgtcggct gctcggtagc cgtggtcaag gactatggcc gtgacgagga ggcgaagaac
      421 gaacttcagg agtacatcag gaagggtgtc aaggcagcgg aataagcccg ccgagaccgt
      481 ctcaagacgt caagagctct ccacagtttg ttgtttcaat ttatgtactg ttgcaagaat
      541 tcgaataaat tgttatttgt taaaaaaaaa aaa
//