LOCUS       CO897946                 518 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09e01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q94045 Q94045 T13F2.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897946
VERSION     CO897946.1  GI:51237736
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 677
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tttatgtcta ccgattcttc ttcctacccg attctggtga agaagaccaa attcccgcaa
       61 aaaaggcaaa gcttcaggag gaaaaacaaa acaaactcgg caaaggaaag gtgtctcaaa
      121 agaagaaagc tctgatcgac tcatcagacg atggctcgaa tggctctgat ggtgaagcgg
      181 tcaaggagaa agccaaagcg aacaagaaga attcgaaggc atcggctgag gatgaatcgc
      241 cagtgcgcga acttgatcaa aacgggaaca agatggttcc gctcggaggc aacaaatttg
      301 tgaccgttag ccgcttcaag gggaaggtct acgtcaacat ccgagagtat taccagaaag
      361 aagggaagtg gcttccagga aagggcgtca gccttacacc agaatatttt cagaatctga
      421 tgcagcatgt tcccgaaatc aagggtctca ttgacgcagc ctgaagaaaa aatacgtgta
      481 tgatgcaatg agtaattgga gttacatctg tttttgga
//