LOCUS       CO897945                 687 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09d12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:H33_HUMAN P06351 HISTONE H3.3, mRNA sequence.
ACCESSION   CO897945
VERSION     CO897945.1  GI:51237735
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcagctaaaa ataatttcaa caatcgaatt cttctgatct tcgctatggc tcgtaccaaa
       61 cagacagcac gtaaatcgac aggaggaaag gctccacgaa aacaattggc tacgaaggcg
      121 gctcgtaaat cggcaccatc taccggtgga gtcaaaaaac cgcatcgcta tcgcccagga
      181 acggttgcac tgcgcgaaat tcgccgttac caaaagtcga ctgaattgct gatccgcaaa
      241 cttccattcc agcgcttggt gcgtgagatt gcgcaggact ttaagactga tctccgcttc
      301 caatccgcgg caatcggagc tcttcaggag gcatccgaag cctatttggt cgggttgttt
      361 gaggacacca atttgtgcgc cattcatgcc aagcgtgtca ccatcatgcc gaaagacatc
      421 cagctggcgc gtcgcattcg cggtgagcgt gcttgagcgg aatctcgccg aacatcgaaa
      481 gctgggggag cattatatgg atgaacccta agaaaccatt ccccgtcacc aatcaaaaaa
      541 ccccctcttc tatgatattt agctaaagtc catcatccaa ggaagaccaa aaaccatcaa
      601 gcacaattaa aatatctctt taaactttgt ctgtctttaa caaacactcg ttgtaaatct
      661 gaaactgaac catttttatg cactcaa
//