LOCUS       CO897944                 516 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09d11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO897944
VERSION     CO897944.1  GI:51237734
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 487.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgcaatggc ttctgaggta gttgggcctt ttgccccact tggaccttac ggtgatcttc
       61 cagatgatga gagttggctc gagactctcc gtcgtgaact tgcgaaatat taccctcaag
      121 atgtcgacac ccagaaagtt cgagaggtat ttgccaatat ttctcaattt agcaaatacg
      181 cagatacgaa agtcaattat ttcttggatt taaacatgtt cgaggaggca aaaaacgcca
      241 cagggcttgc acgtttcgac acaaagtctt ttcttcgcat cctcggagca cttgtggcgt
      301 tcaaggagtt ccgcgagatg tatgacatta gccaacaggc tgctaaagag attagtcgcg
      361 cttgtattga caagatacaa gatcattttg atgaatatgc gatcgacgtg ttcaaaattt
      421 ggaaacacta caacgaataa actttcctct caaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 aaaaaaanaa aaaaaaaaaa aaaaaaaaaa aaaaaa
//