LOCUS       CO897933                 483 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09c08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL27_CAEEL P91914 60S RIBOSOMAL PROTEIN L27, mRNA
            sequence.
ACCESSION   CO897933
VERSION     CO897933.1  GI:51237723
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 568
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcttgctact tccattttag aataatggga aagattatga agccaggtcg ggtagctatt
       61 gttctcaatg gccgttttgc cggacgcaag gcagttgtcg taaagagcta tgacgaaggc
      121 tcaaccgatc gtccttatgg acatgcgctt attgccggga taaacaagta cccattgaaa
      181 gtgacaaagc gaatgggcaa gaaaaaagtg gcgaagcgga tccgcatcaa gcccttcata
      241 aaggtcgtct cgctgcagca tctgctgccg actcgttgca ttttcgatgc tgatttcgac
      301 aaaagcgccg tgaacaagga gtcaattaag gagccaagaa agaagaaaaa tgcgcttagc
      361 aacgtgaaga aagagcttga gacgcactac aaaactggca aaagcaagtg gttgttcaca
      421 aagctgcgat tctaagggaa ttttgtggtg aaaagggaaa gaatgctgtt gttgttaaac
      481 att
//