LOCUS       CO897920                 556 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09a11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O61880 O61880 F59B1.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897920
VERSION     CO897920.1  GI:51237710
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 612
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aagatggcca agtccgttgt cgttctcctc gtcctgtcgg ccggcatgct gttgacggag
       61 gcaaagaagc agcagcaagc ggacaaaggg ccgcaatccc agccagtgcc acgaccatcc
      121 gagcaacaga cccagcagca aaacgagggc ccgaatcaac agccgggcca gttctgtgtg
      181 aagccgccga agaagcccaa gagcctgaag gtgctgacgg agtacgagca gtgcaaacag
      241 gagtgcaaga agaagcgaga ccaggagaac gccgggcagt acgtggaatg gctgagggag
      301 gagctgcgtg tggcggagca gctagcacag gaacaggcgc aggcggccgc ccagcaagag
      361 gccccagaac aagcggttta gccgagttga gaaatttcca gaccaaaggt cttccttcaa
      421 gtggtttttg aattgaaaat tctttcgact tcttgatcag taaagagggg tcgaaagggc
      481 cagaattgta aagaataaag aaccctttgc cgaacaaaca aatatttggt cccctaataa
      541 agtggatttt attttc
//