LOCUS       CO897919                 507 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09a09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS17_CRIGR P06584 40S RIBOSOMAL PROTEIN S17. [1],
            mRNA sequence.
ACCESSION   CO897919
VERSION     CO897919.1  GI:51237709
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 532
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttcgcgaaaa taaaaatggg ccgagtccga actaagaccg taaagaaggc ttcgcgtgtg
       61 ataatcgaga aatattacac gaagctgggt cacgacttcc acaccaacaa gcgcatctgt
      121 gaggaggtgg ccgttattcc gagcaagcga atgcgcaaca gaattgcggg atttattacc
      181 catttgatga aacgtattga gaaaggtcca gttcgaggca tttctatcaa gttgcaggag
      241 gaagaacgcg aacgtcgtga caattacatg ccggatattt ctttcctgga tgcaactctt
      301 cattctagca tcaacgtgga tgccgagacc aaagagatgg ctgagaagct gggccttgga
      361 ctgaatctgg tagtcgattc gaagactgca gccggaggcg gcgggggccg acgtcaatga
      421 acgcagtgaa cgaagaaatc gatgttgaac cgaattgtta ttgttatttt atacggcaat
      481 tgcgataatt gaaaaaaaaa aaacaaa
//