LOCUS       CO897915                 400 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09a04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O18694 O18694 F28F8.3 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897915
VERSION     CO897915.1  GI:51237705
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 427
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 taataatggc gtcaactaca acaaatccat cgacgttatt gccattggaa ctaatagaca
       61 aatgcgtcgg ttcaaaaatc tgggtaatca tgaaaaatga aaaagaaatt gttggcacac
      121 ttgtcggttt tgacgactac gtgaacatgg tcctggaaga cgttgttgag tatgaaagca
      181 cctccgaagg gaagcgagtc acgaaattgg acacaattct gctgaacgga aatcacataa
      241 caatgctagt tcctggcgga gaaggacccg aaatctaatc actactttag agtgaattca
      301 gatctcaatt ttacacacac aaaaaaagga agttgttgct ttatgttttt ataattttgg
      361 ataaaatact cgatcgcaaa aaaaaaaaaa aaaaaacaaa
//