LOCUS       CO897914                 448 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe09a02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS1A_HUMAN P39027 40S RIBOSOMAL PROTEIN S15A. [1],
            mRNA sequence.
ACCESSION   CO897914
VERSION     CO897914.1  GI:51237704
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 483
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 caaaatggtt cgtatgaatg ttcttgctga cgctttaaag tcaatcaaca atgccgaaaa
       61 gcgaggaaaa agacaggtga ttatccgccc gtcttcgaag gtgattgtgc gctttctcac
      121 cgtaatgatg aagcacggat acattggcga attcgaaatt gtggatgacc accgagccgg
      181 taaaattgtg gtcaacctga ctggacgaat caacaaatgc caagtcatta gcccgcgttt
      241 cgacatccaa atggccgact tggagaagtg gaccagcaac ttgcttccgt ctcgccaatt
      301 cggctacatt gtgttgacca cgtccggagg aattatggac catgaggaag cgcgcagaaa
      361 gcacatggga ggcaaaattc tcggcttttt cttctaaacg ttgttgctcc cttgaatttt
      421 gtttattgct attgttgaat gttgagga
//