LOCUS       CO897900                 388 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08g07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P91302 P91302 SIMILARITY TO ARABIDOPSIS THALIANA
            UBIQUITIN-LIKE PROTEIN 8. [1], mRNA sequence.
ACCESSION   CO897900
VERSION     CO897900.1  GI:51237690
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 412
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aaatcagcaa cagcagttat gattgaaatc accgtcaacg acaggttggg aaaaaaggtt
       61 cgaatcaagt gcaatccaac cgacacaata ggcgacttga agaagctagt cgcggcacaa
      121 attgggacaa ggtcggacaa gcttgtgctc aagaagtggt acacaactta taaggatcac
      181 atcacactcc aggactatga aattcacgat ggctttaact tcgagctgta ctaccaatga
      241 attgctgtgg tgttaatgga tcagcagaaa attactgaag caaccgtttc gaagttcatc
      301 atcattcgtt cattaacttt cttttttata atttatataa aacataatcg gaaaaaaaaa
      361 aaaaaaaaaa aaaaaaaaaa aaaacaaa
//