LOCUS       CO897896                 493 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08g02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL27_CAEEL P91914 60S RIBOSOMAL PROTEIN L27, mRNA
            sequence.
ACCESSION   CO897896
VERSION     CO897896.1  GI:51237686
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cttccatttt agaataatgg gaaagattat gaagccaggt cgggtagcta ttgttctcaa
       61 tggccgtttt gccggacgca aggcagttgt cgtaaagagc tatgacgaag gctcaaccga
      121 tcgtccttat ggacatgcgc ttattgccgg gataaacaag tacccattga aagtgacaaa
      181 gcgaatgggc aagaaaaaag tggcgaagcg gatccgcatc aagcccttca taaaggtcgt
      241 ctcgctgcag catctgctgc cgactcgttg cattttcgat gctgatttcg acaaaagcgc
      301 cgtgaacaag gagtcaatta aggagccaag aaagaagaag aatgcgctta gcaacgtaaa
      361 gaaagagctt gagacgcact acaaaactgg caaaagcaag tggttgttca caaagctgcg
      421 attctaaggg aattttgtgg tgaaaaggga aagaatgctg ttgttgttaa acattggatg
      481 ttttcaattt tta
//