LOCUS       CO897895                 518 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08f12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:CYC1_ASCSU P92504 CYTOCHROME C, TYPE-1. [1], mRNA
            sequence.
ACCESSION   CO897895
VERSION     CO897895.1  GI:51237685
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 555
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgagaactgc caatggccga aattcctgag ggtgactacg agaagggaaa gaaagtcttc
       61 aaggcccggt gtctccaatg ccacgtgatc gactccatgg ccacaaagac aggaccaacc
      121 ctgaatggga tttggggaag gaaaagtgga caggtacccg gattcgacta ttctgccgcc
      181 aacaaaaata agggtgttgt ttggaatcgc gaaacgctct tcgaatacct gctcaatcca
      241 aagaaatata tcccggggac gaagatggtt tttgcagggc tgaagaaggt cgacgaaagg
      301 gcggatctca tcaagtacat cgaggttgaa agtcagaaac cgccagcatg atgaattgac
      361 gcatgagagg ttcatcgccc gtccacgaca aacaggatag tttatagtca gtcacgtgcg
      421 agacacttgg ttaaaattgt ttttcaccta gacattttca cactctgagg ccctcgtttc
      481 taaaaaattg ttagttcgaa aaaataaaat tcgttgac
//