LOCUS       CO897894                 533 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08f10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93622 Q93622 F28C6.8 PROTEIN, mRNA sequence.
ACCESSION   CO897894
VERSION     CO897894.1  GI:51237684
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 519.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 taatggtact aagtggcttt gcattctatc cgacacttgc ttacaattta tttcgaaatt
       61 atgttcaacc aaagagttgg agttggtata cacgaattaa cgaatcactg attttgggcg
      121 caatgccttt caactcaatg ttactggaac tcaagacaga ggaacaggtt ggaggagtgg
      181 tttgttgcac agaggaattc gagctcgatg cagcttgggg ggcacttaaa tcagccgatt
      241 gggcacatca tggcattgaa tttcaccata cgccaatgaa agacttcacc ggttctacct
      301 cgcagcgaag catagcagag gcagttcatt tcatcgaccg aattactgca gagggcaaga
      361 cttgctacgt ccattgcaaa gctggtagaa cacggagtgc cactgtggcc atttgctacc
      421 taatgcaaaa atacgaccat agccctgaag aggcaatgaa tatgcttaca ttgaagcgcc
      481 ctcaggttct tctacgtgaa atgcattgga gatctgttcn agagttcagg aag
//