LOCUS       CO897890                 471 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08f06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9U8J7 Q9U8J7 PEPTIDYL-PROLYL CIS-TRANS ISOMERASE,
            mRNA sequence.
ACCESSION   CO897890
VERSION     CO897890.1  GI:51237680
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atctcgtcgc gtattttgca cactgggaag caattgagaa tgccggtcca aattgacact
       61 ctgaaagagg gagaccagtc gactttcccc aagaagggcc aaaaggttca atgccattac
      121 gtgctgacgc tggagaacgg acaaaagatc gacagttctc gcgatcgcgg aaagcctttt
      181 gaattcacga ttggaaaggg cgaagtgatt cgcggctggg acgagggagt ggccaagatg
      241 tcggtcggac aacgcgccaa actgaccatc agttccgaca tgggctacgg accgaaggga
      301 attccgggaa ccatccctgg caatgccact ctgctcttcg acgtggaact gctcggagtc
      361 aagtaaacaa ctggctgatg aagagaatga gccctctcaa gacggagatg acccatctat
      421 cgtttctttt cttagtgtta aataaattgc cactgttttt taaaacgccc a
//