LOCUS       CO897885                 633 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08f01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9XVF7 Q9XVF7 B0250.1 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897885
VERSION     CO897885.1  GI:51237675
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 543.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtttaaaata atatcaaaat gggccgtcgc atccgagcgc agcgaaaagg gcgtggagga
       61 atcttcaagt cacacagcaa gcatcgcaag ggacctgcaa aattgcgtcc ggtggactac
      121 tcggagcgtc acggctacat ccgaggaatt gtcaaagata tcatccatga cccgggtcgt
      181 ggtgcaccgc ttgctattgt gcaattccgc gacccataca aatatcgggt gaagaaagag
      241 acaatgattg cggccgaagg attacacaca ggacagttca tttattgtgg aaagaaagct
      301 caagtccaaa ttggcaatgt gatgccgata ggcgaattgc ccgaaggtac cactgtgtgt
      361 aatttagaag agaaagtcgg agatcgaggc cgtctggctc gaacttcagg caattatgcc
      421 atcatcatcg ctcacaaccc agagagcaaa cgtactcgta ttcgtttgcc atccggtgcg
      481 aagaaggttg ttccatccgc caaccgcgcg atgatcggga ttgttgccgg tggcggtcgt
      541 acagaccagc cgttgctgaa aggccgaacc ccccaacatc tttacaaggg ctaaccgcac
      601 gcttgcccaa aatgcatggg tgtgacaata aac
//