LOCUS       CO897880                 565 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08e06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE25457 Y54G2A.O, mRNA sequence.
ACCESSION   CO897880
VERSION     CO897880.1  GI:51237670
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgattactga tattatcaat tatcatttaa tattagacta cattcaagaa aatggcattg
       61 ccacgacgca tcacaaagga aactcagaga ctgctccaag accccgtccc gggaatttcg
      121 gccataccgg atgaggccaa cgcacgctac tttcacgtta tcattgcggg tcctgatgaa
      181 agcccatttg ccggtggcat tttcaagcta gagctgtttc tgccagagga atatccgatg
      241 gcggcgccaa aagtgcgctt catgacaaaa atttatcacc cgaacattga caagcttggc
      301 cgcatctgtt tggacattct caaagacaag tggtcgccgg cattgcagat ccgcactgtg
      361 ctcctttcaa tccaggccct gttgtcggcc ccgaatccgg acgacccgct tgcaaccgac
      421 gtggcggagc agtggaaaag caacgagact gaggcgatcc aaatcgctcg cgaatggaca
      481 cgactctacg cgggcgagaa ctgagcattg gccagggcac aggaaaataa gaggaataaa
      541 aagaataaat tgacaattga ctgtg
//