LOCUS       CO897875                 446 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08d12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE00739 F37C12.4 Ribosomal protein YL39, mRNA
            sequence.
ACCESSION   CO897875
VERSION     CO897875.1  GI:51237665
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 483
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gacgaagcac tccgctgtcg aggcccttgc tgtgggactg gagaaaggct atccggtgac
       61 aagaaatgag cgcaagaagc gtcagtcgcg gcacaagggt cgcctgtcga agaagactcg
      121 cattgtgcgg gaactggtgc gcgaagtggt cggctttacg ccatatgagc gtcgcaccat
      181 ggagctgttg cgcatcagca aggacaagaa ggcactaaag ttcctgaaga agcgcattgg
      241 acagcacgga cgtgccaaga ggaagcgtga cgagatccaa caggtgttgg tggcgcagcg
      301 caaggcacac aaatgagaaa gagtgcgcga tttgggatca atccaattat tgttatgctt
      361 gttgagatac aaaatcgtaa aaaacgcatt ttcaggaata aaaagtgccg aaatataaaa
      421 aaaaaaaaaa aaaaaaaaga aaccaa
//