LOCUS       CO897874                 628 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08d11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:BTF3_CAEEL Q18885 TRANSCRIPTION FACTOR BTF3
            HOMOLOG. [1], mRNA sequence.
ACCESSION   CO897874
VERSION     CO897874.1  GI:51237664
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgggtttacg ttcgagtttt atagttggtt tttataattt gtttattggg ctctcctatt
       61 cgttcacaac ctacattgcc gtttaaaatg aatgccgaga aagtaaagaa gcttcaacaa
      121 aatgccgagc atgttcgtac aggaggaaag ggtactgcac gtaggaagaa gaaggtggta
      181 cacaagacca ccgcaatgga cgataaaaaa ttgcaaagca acctaaagaa actctctgtg
      241 accaacattc cgggtattga agaagtcaac atgatcaagg acgatggaac cgttatccat
      301 ttcaacaatc cgaaggtgca agcctctgtg ccagcaaaca ctttttcaat cactgggact
      361 gcggaaaaca aacagatcac cgacatgctg cctggcatcc ttaaccagtt gggcgctgaa
      421 tctttgactc acttgaagcg gctggccaac aacactaccc aattcaaagc cactgaagat
      481 gacgatgtgc cagatcttgt cggagatttt gaggaagctt caaagaacga gtcnnagacc
      541 accgggactg ctgcggacac caatgttaat gccgtcgaat aaaatagcca atttgctgtt
      601 gacgatggaa caaattgatc agattttc
//