LOCUS       CO897872                 490 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08d09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01542 O01542 CODED FOR BY C. ELEGANS CDNA
            YK66C9.5. [1], mRNA sequence.
ACCESSION   CO897872
VERSION     CO897872.1  GI:51237662
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 526
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcgtctcgc actactgccg gaggtattgg tttccaggtt gtggccaagc aggcgagcaa
       61 atacagcaac caggagggag agatgtgcct ggcctggatc aagaaaatga gcggcgagaa
      121 tatcagcacc aacggcgacc gtgagaactt ctacaaattg ctaaaagacg gcacactact
      181 gtgcaagctg gccaacggga ttcaggcggg ctccgtcaag aagatccagc agccgactgg
      241 caacttcgcc tgcatggaga acatcaacgc tttcgtgtcg ttcgcgaaga gtagaagtga
      301 gtgcagcaaa agacggacag agcatgtgct atcgctccca ttcgagaact ccccgttttg
      361 cgaattttct ccccgtgtgc gaccttgcaa caataccgag gcgttaactg ttttcccctc
      421 tctctcctct caaactgtgg gcatttgaaa atagcgatgc cggaataaat ggccaattca
      481 agaaaaaaaa
//