LOCUS       CO897860                 484 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08c04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O64955 O64955 POLYUBIQUITIN, mRNA sequence.
ACCESSION   CO897860
VERSION     CO897860.1  GI:51237650
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 561
            Seq primer: SL1 primer
            High quality sequence stop: 481.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gactattacc ttggaagtag aaggttccga cactatcgag aatgttaaag ccaaaatcca
       61 agacaaagaa ggcattccac ctgaccaaca acgattgatt ttcgccggta agcaactcga
      121 ggatggacga actctagccg attacaacat ccaaaaggaa tcgaccttac acttggtttt
      181 gcgccttcgc ggaggaaagg tccatggttc gcttgctcgt gccggaaagg ttcgcgctca
      241 gacaccaaag gtcgaaaagc aagaaaagaa gaagaaaaag cgcggacgtg cattccgtcg
      301 tattcaatac aaccgtcgct acgtcaatgt tgcaatggct ggaccaggcc gcaaacgcgg
      361 acccaactcg aatgctgcgt aacttggaaa agattaaatg gattcattcg catgaattga
      421 tgtttttgca ggaatccttt ttattttctg ttatttgaaa ttcgataaac caatttgttg
      481 caaa
//