LOCUS       CO897855                 540 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08b09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q20901 Q20901 F56H11.4 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO897855
VERSION     CO897855.1  GI:51237645
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcccgcttct gcccaatgcc ggctgactcc gttccggtcc cctccctgtg ggagaatgtg
       61 actgcggcgc cgcacttcga gccggccacc ttcttccact tgctcaccgc cgccgagttc
      121 ccagagccgg aggctcgcaa atggattgac gcgcggtttc ccctgaccat ccagatctcg
      181 atcgcttacg tgctggtggt gttcggcacg cgcttcctgc actggtacca ccacatcctg
      241 accatgctgt acgccttcta ctcctacccg atcacgcccg ccttcaaccg ctggggcatc
      301 ttcctcaact atctggtcca ctcctacatg tacacgtact acttcttgcg ctcaatgaaa
      361 atccacgtcc ccggcccggt ggccaaattc gtgacgacca tccagatctg gcagttcatc
      421 atctcggtgg ccatcctctg ccacctgggc ttcatcgttt ttgcccgaaa cgtgcaatgc
      481 gacctggacg ggcgggtgtt cgcgttcgcg gtgggcatgg acgtgtccta cctggtgctg
//