LOCUS       CO897845                 308 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08a08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YOY2_CAEEL P34660 HYPOTHETICAL 6.3 KD PROTEIN
            ZK652.2 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO897845
VERSION     CO897845.1  GI:51237635
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 390
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggaagcgatg gacatgtata atccggccgc cgtcggttcg acacaggtgc tctcgagacg
       61 tgaaaaaatg ctctctacat cggctgacgc attcaagttc acgctgcaat ggggtttcgt
      121 gccgttcgtt gtctatttgg gttttcgccg tggcgctgat cctctcccac atggaagcat
      181 tccgccgctg tcgttttcca gtttgttgtg gggatagacc gcaaacagaa gaacggattg
      241 tggaacaagc atccactggt agactgtaat gtttctagca cattgtttta tttgttttga
      301 cagtctgc
//