LOCUS       CO897841                 540 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08a04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01868 O01868 CODED FOR BY C. ELEGANS CDNA
            YK71B8.5. [1], mRNA sequence.
ACCESSION   CO897841
VERSION     CO897841.1  GI:51237631
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 621
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctaattttac gcgtaaaata gcgataatga aaactgagac ctgtgtttac tctggataca
       61 agatccaccc tggacatggc aagcgcatag tgcgggcaga tggaaagatt caaatctatc
      121 tgagcgctaa atgccaacgc ggtgctgcac tcaagcgcaa cccgcgtgat gtgccatgga
      181 ctgtgcttta ccggcgcaag cacaagaagg gtgtgcacgc ggaagaaggc caacagaaga
      241 agcgcatgaa gcgcactgtc caaatagtct ctcgacccat cgccgatttg actgtggaag
      301 cgttgttggc acagcgaaac cagaagcctg agttccgcaa acagcagcgt gatgcagcga
      361 tcaaagcggc caaggaatct gtacgcgcaa agaaagagga aaccaagaag aaggccaaac
      421 tggacaaaaa ggcggcaccc caaaagatga aggcggctaa gcctgtgaaa gccccaaagg
      481 cacaagttgg gggcaaacgt tgaaaagagt ttgattgttg agtgttgatc caatttgccg
//