LOCUS       CO897840                 526 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe08a01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL15_RAT P41051 60S RIBOSOMAL PROTEIN L15, mRNA
            sequence.
ACCESSION   CO897840
VERSION     CO897840.1  GI:51237630
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 402.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttgaaattgt tgagaaagca tcatgggcgc atacaaatac atgcaagaga tctggcgcaa
       61 gaagcagtcg gacgtgatgc gctacttgct gcgcattcgc tgctggcaat accgccaaat
      121 ggctgccatt caccggtgca gccggccgac ccgtccggag aaagcgcgtc gtctcggcta
      181 caaggccaag caaggttacg tcatctatcg tgtgcgcatc cgccgcggtg gccgcaagcg
      241 tcaagtgacc aagggtcaga cctacggaaa gccgaagaca cacggagtga accagttgaa
      301 gaacacgcgc agtcttcagg ccgtcgctga agcacgaatt ggacgtcgtt gtggagagct
      361 gcgtgtgctc aactcttact gggtgggcca ggactccacg tacaagttct acgaagtgat
      421 cctggtcgat ccgtttcaca aggttattca gtgcaaatcg ggaacacaat gaattacaaa
      481 actggtgcaa aaacatcgta aaaaaacggg aactcatacg gacggg
//