LOCUS       CO897828                 505 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07g01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q94539 Q94539 PUTATIVE RNA-BINDING PROTEIN. [3]
            TR:P91680 TR:O02392, mRNA sequence.
ACCESSION   CO897828
VERSION     CO897828.1  GI:51237618
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcaactaaac cttaattggt caacatcttc tcaaatctca tctatatgac tatggactat
       61 cttgctgaac tcatccgtga aaagagcact gtggcggcat ttcccaatca cttcaaacat
      121 gttgatcgct taatagactt ggaaattgct cgagttcgcg ctcagctctt ccaatgcagc
      181 tttgacttct ctgagctctt gaagcacatc gatgcctgaa tccgaatcca ccttctatca
      241 cgatattaat catatcgttg cagtcgtttt tgtctctaaa ttaagtggcc tctgtctctc
      301 tctctctctc tctctctgcc catctaatcc cccttcaatc tctctatcgg catttccctt
      361 aagtcgaggc tataatccaa aaaataagtt ttacccccca taatcgataa tttaaagtaa
      421 tttaagttgt ttcgcttaag cgataattaa tgttcacgca taaattggtt gtttgttgtt
      481 gccctctcac gatgatactg ttgaa
//