LOCUS       CO897817                 432 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07e12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21265 Q21265 SIMILAR TO METALLOPROTEINASE
            INHIBITOR 2 PRECURSORS. [1], mRNA sequence.
ACCESSION   CO897817
VERSION     CO897817.1  GI:51237607
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 atgtgtttgt tcggcaccgc cattttgttc tgctcgctgc actattcgtc cttctgccgt
       61 ttccccgagt ggagacgtgc aagtgcaagc tgtcttcgtt caaccaaacg atgtgtgagg
      121 ccaaatgggt ttcccatgtg agcgtgctga acaacgagac cactgcccac tccgtcaact
      181 acactatgaa gttcgtcgaa gtgttcaagg cgcccaacgg gactcagatg gcgggttcca
      241 ctcgcagagt gctgaccccg ctgcacgacg ccgcatgcgg ggtgtcggat ctgcagccgg
      301 acagggacta cttgttggcc ggcaatgtgg acgatgggga cctgctgcgc atcattcgct
      361 gcctgtacgt accgctagcc gatggaagcg ggaatcctat ggccgctcgc gattgggacg
      421 cagtaccgga cg
//