LOCUS       CO897809                 523 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07e03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS3A_CAEEL P48154 PROBABLE 40S RIBOSOMAL PROTEIN
            S3A. [1], mRNA sequence.
ACCESSION   CO897809
VERSION     CO897809.1  GI:51237599
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 416.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcggtcgga aagaacaaga agatgggcaa gaagggtgct aagaagaagg cggttgaccc
       61 gttcaccaga aaagagtggt acgacatccg tgctccggca atgttccagc accgaagtgt
      121 tggcaagaca ctcgtgaacc gaacccaggg aactcgcatt tcaagcgatt ttttgaaggg
      181 ccgtgtctat gaggtttctt tgggtgatct taacaacact gatgccgact ttcgcaaatt
      241 ccgcttgatc tgcgaagaag ttcagggcaa gaattgcttg acgaacttcc atggaatgac
      301 tttcactcgg gacaagctct gctcgattgt caaaaagtgg catacgctta tcgaggctaa
      361 tgtggcagtg aagaccacgg acggctatat gctccgtctg ttctgccttg gcttcactan
      421 acgtagcctc aacccgattc aaaagacccg ctatgcgaaa acttcgccag tgcgcgccat
      481 tcgggcctaa atgggggaga aaatgcaaaa ggaggtgtcg tcc
//