LOCUS       CO897806                 513 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07d11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9U1P3 Q9U1P3 UBQ-2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897806
VERSION     CO897806.1  GI:51237596
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 564
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctgtcgactg aatatcacca attctaaaat gcaaatattt gtgaaaacac tgaccggaaa
       61 aactatcaca ctcgaagtcg aggggagtga cacaatcgaa aatgtaaaag cgaagatcca
      121 ggacaaagaa ggaattcctc cggaccagca acgtctgatt ttcgcgggaa aacagttgga
      181 ggacggacgt actctggcgg attacaacat tcagaaggag tccactttgc acttggtgct
      241 gcgtctccgc ggaggaatca ttgagccgtc gctgcgcatg ttggctcaga agtacaactg
      301 tgacaagcag atttgccgca agtgctatgc tcgtttgcct ccgcgtgcaa ccaactgccg
      361 aaagaagaag tgcggtcact ccaacgacct ccgcgtcaag aagaaactta agtgaagaag
      421 cagatggatt ggcggaagag ccgaagatgt ccatcgctat tttgagctgt taatcgttca
      481 ccattgttga cgtataaatt ggacgtaaaa aaa
//