LOCUS       CO897796                 514 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07c09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO897796
VERSION     CO897796.1  GI:51237586
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 522
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 actcacctct cggacgcttt agggcacgag agcgaacttg gacgagtcga gcggcgccca
       61 tctcgactgc acggcgaatg gcgttcatcg catggaacag cacccccgag gaggtggatg
      121 cgcccgccga agcggaggaa ccgaacaaca atggaacaat gcaaactggg agcagcgctg
      181 aggaagaagt gaagagcgcc attcggccaa cagacggaac aacaacaaca gagcaagaag
      241 gaaacggacg aacaacaaaa acaaagcaca gccgcagagt ctttctcgac gcattcggca
      301 cccccagcag agtctttcac aagccattca ttttcggtct ttcatccccg caatccacca
      361 tttgttgccc gcaccccaaa agcccaaaaa cagaactaca aacacacaca aacacaattg
      421 tcgcggtcag cactgtgccc ggaggactgt gaccaaaaca tgccctaaat gtgcgaatga
      481 ataaaacgga ttcaccacaa aaaaaaaaaa caaa
//