LOCUS       CO897789                 458 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07b10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9ZPP6 Q9ZPP6 LIM DOMAIN PROTEIN PLIM-2, mRNA
            sequence.
ACCESSION   CO897789
VERSION     CO897789.1  GI:51237579
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 482
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgcagctgag ccagacatca aggattgtgc gcagtgtggc aacaaggtcg agcacactga
       61 taaaatgatg gtggacaggg agacagttca caaagactgc ttcaaatgca gcctttgcgg
      121 cacaaaactg caggtgggat tctgcgccat ggatcaagcg ctctacgggc gctatggacc
      181 ccgctggtat tgctccctga tctgcgctca tgaaccgctc ggcaagaagg aggcaaaact
      241 gaaggagcag ggcattgagg tcagaaagcc gaaatcgaaa aattaattga gccgcagaga
      301 agagagtgga aggaagcaac aatttgccct gttgaactgt gaattgaaac aataacaagt
      361 gccagtaata ttttatgcag atgctcttcc tattcctaac gaataaattt tttagaataa
      421 tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaacccaa
//