LOCUS       CO897788                 661 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07b09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE24630 Y71H2B.3, mRNA sequence.
ACCESSION   CO897788
VERSION     CO897788.1  GI:51237578
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 581.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgagcgcacg ctcgaacagg aatttactta ttgcgaagga accatagagg atctgattgc
       61 tgatcgactc ggggattcga gattacctga aagaaatgag agactgaaga attgcgtgca
      121 tacactgcag tctctaaacg atctcacaaa tgaactttct ctgttcagca caaatgacca
      181 ggctgaagag ttgccaacga gttcacttcg ttacctgctc ataccagttt atctggccta
      241 tacaatgcaa gagatacaag tgggaatgga tgagcgcaaa tcctacctca aagcagcgaa
      301 gtcatactat cgggaatatc tgcaaaacct tttggttttc gagctcatca actttaaact
      361 tccttggatt gattcggaga gaggcgataa agaaggcaat tctggtatcg atattccgcc
      421 agttagcggt ttcaaagaag cgaccgcccg acgagagaag aaactaactc gtttgaatca
      481 aatgaccgag cttgagaaag cgctcgtgaa acttcggatt gaacatacac gaaacgatga
      541 cgaatttaca caaagagaat tgataataac tctcttgcgc ctaagggcta ttggcactca
      601 tcacgaactc gataaatttg aagaagaact aacgttactc gaacatttgg aaacgagatc
      661 g
//