LOCUS       CO897787                 457 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07b07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9XVE9 Q9XVE9 C04F12.4 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO897787
VERSION     CO897787.1  GI:51237577
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtattcaat tattcttgga cgcacaacat gatctaccac aaatttgtcg agatcggacg
       61 cgtggtgtac atcgcaaagg gcaagaagca gggcaagttg gccgtgatcg tgaacgtcat
      121 tgacgggaac agggcactgc tcgacggtcc ctcgtccgga gtgccgcgcg gcgtgtggaa
      181 cttcaaagag ctgcagctga ccaagtacaa gctgacgctg cgcgtcggcc agcgcaccaa
      241 gggcgtgcaa aaggcctacg acgaggccaa gatcaacgag cagtgggccg actcttcgtg
      301 ggcaaagcgc atcgccagca aagcaaagcg cgccagcctg aacgacttcg agcgcttcaa
      361 ggtgttcaag gcgaancaga tgcggaaccg cattgtgcgc acggagctcg gaaagctgaa
      421 gaaagcgacc aaaaagagtg gcgccgcccc aatggcg
//