LOCUS       CO897782                 468 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07b02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90976 P90976 LIN-7 ;contains element TAR1
            repetitive element, mRNA sequence.
ACCESSION   CO897782
VERSION     CO897782.1  GI:51237572
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 547
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgtcagagaa catagctaat aagccaacaa attgacaatg atatagagca gataatcgtg
       61 atgttgctgc gtagattgaa tctgtcgccg tctctgacct tttaccgtta taaaatgaag
      121 cccccctcga acatccccta ctccggcatc taccggacca atggcgacat ctgcagaagg
      181 gacgaactgc tggtgtgtca acggaagatg aactatcacc ccggcgccaa tgtccgtcac
      241 cagccagatc ggggccaaca cttgctgaag gcgaactgcg acgggactgt ggtgatcaca
      301 cgcgagcact gccaagtgga cgagaacaac gaatggatgc aaaagttcta cgcacatcgt
      361 aactttgagc acatctacaa actcacgttc aatgttatcc cccgtaagat gtctacttca
      421 tttactctag ttagcacaga gtgaataaat ttattttact aaaaaaaa
//