LOCUS       CO897780                 482 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe07a11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9VT06 Q9VT06 RPS9 PROTEIN, mRNA sequence.
ACCESSION   CO897780
VERSION     CO897780.1  GI:51237570
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 448.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gagtaaagca cgtattttct aaagtctatg acgtgcctcg tcgtccattc gagagagagc
       61 gacttgatca ggagctcaaa ctcattggtg agttcggtct cagaaacaag cgagaggtgt
      121 ggcgtgtcaa actgacgctg gccaagatcc gaaaggcggc ccgtgagctg ctgactctcg
      181 aggacaagga ccaacgacgc ctgttcgaag gcaatgcgct gctccgtcgt ctggtccgag
      241 ttggcgtgct gcccgaggac aaaatgaagc tcgactacgt gcttggtctc cgcgtcgagg
      301 acttcttgga gcgtcgtttg cagacacagg tgttcaaact cggactcgcc aagtccatcc
      361 accacgcacg tgtcctcatt cgccaaaagc acattcgtgt gcgtcgacag gtcgtgaaca
      421 tcccatcgtt cattgtgcgt ctggactcgc agaaacacct cgacttttca accaagtctt
      481 cg
//