LOCUS       CO897766                 489 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe06f12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:NUFM_CAEEL Q18359 PUTATIVE NADH-UBIQUINONE
            OXIDOREDUCTASE 17.3 KD SUBUNIT, mRNA sequence.
ACCESSION   CO897766
VERSION     CO897766.1  GI:51237556
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 473.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tatcgtcttt tgctcggcaa cacgttgctg gcacatccgc gaccacgtgt tcaaaaaagc
       61 gagcgattcg tttcacgcga tgaaggtggc tacgcgactc agatgttcga gaactattgg
      121 gtgcgccaac accagaaaaa gcgcgacaac gtttcgccgg actttcacaa aaagactaca
      181 ggaatgtgcg gcctgtacgt gaatgagcac ccgcaccacg acttgaaggc gctgtacaag
      241 cgccttttgc gcgtgctcgc catgatacca gagcaatcgc tgtacaggca gtccaccgag
      301 gagattgtga agcaacgtct cgcgcacgtt gaacaggaac cggacatcca gaagctggaa
      361 gagcgcatcg ggatgggcca aattgaagag gtcattgaac aggcggagaa cgagttgcaa
      421 accgcgcgca ttttcgtcca acatcaggct tgggagcctt tggtggaagc acctctggaa
      481 taacaatgg
//