LOCUS       CO897759                 518 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe06e11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9VP65 Q9VP65 CG12975 PROTEIN. ;contains element
            TAR1 repetitive element, mRNA sequence.
ACCESSION   CO897759
VERSION     CO897759.1  GI:51237549
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 503.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cttgcaaatg aagctgttga tgcacaattt cttctcatcc cacttcctaa agggtgtgaa
       61 taccggttat ccgctgatat tgcgtgcaac caaaattgag agagtggacg ccaacttcaa
      121 cgcagagttc gttaagaaga tgatgccacg cctggactac caggctcttt tgcaagctgc
      181 caaggtggtg gaccagagcg cagtggcgga actgcccgaa gagctaaacg tcgagtggga
      241 gaaagacgag gagctgctaa aaaagataca ttcgctgttg atgagctacg agattgtgga
      301 gggcgagctg gagtgccccg aaacacatca cgtgttcaac atccgagagg gcattcccaa
      361 tttcctggtc ggcgctgacg aggtcgccta ggattcggac cttggaatca aggtctgaag
      421 gcaaattcca tgtttctgtt cccaataatc catctctgca attttttgag tttatatttg
      481 agtccgcatt aaacacatat tcanaaaaaa aaaanaaa
//