LOCUS       CO897735                 425 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05h06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O61880 O61880 F59B1.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897735
VERSION     CO897735.1  GI:51237525
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agatggccaa gtccgttgtc gttctcctcg tcctgtcggc cggcatgctg ttgacggagg
       61 caaagaagca gcagcaagcg gacaaagggc cgcaatccca gccagtgcca cgaccatccg
      121 agcaacagac ccagcagcaa aacgagggcc cgaatcaaca gccgggccag ttctgtgtga
      181 agccgccgaa gaagcccaag agcctgaagg tgctgacgga gtacgagcag tgcaaacagg
      241 agtgcaagaa gaagcgagac caggagaacg ccgggcagta cgtggaatgg ctgagggagg
      301 agctgcgtgt ggcggagcag ctagcacagg aacaggcgca ggcggccgcc cagcaagagg
      361 ccccaaaaca agcggtttag ccgagttgag aaatttccag accaaaggtc ttccttcaag
      421 tggtt
//