LOCUS       CO897733                 521 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05h03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:TCTP_CAEEL Q93573 TRANSLATIONALLY CONTROLLED TUMOR
            PROTEIN HOMOLOG, mRNA sequence.
ACCESSION   CO897733
VERSION     CO897733.1  GI:51237523
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 507.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 acatcttcac tgaagatgag ctcgcctccg actcattccc tatgcaactt gttgacgatc
       61 ttatctttga attcaagggc cgccacgttg tgagaaaaga aggagaactg tccctggacg
      121 gcgcaaatcc atcaacagaa gaacaagatg agggaactga agagcatgtg gaacgcggaa
      181 ttgatttcgt tctcaaccac cggcttcaag aaatgaactg ctacgaagac caggctacgt
      241 tcaaagcata catcaaagac ttcatgaaga aagttatcgg acatctggaa aaggatggca
      301 aacccgccag tgatattgat gccttcaaga agaagatcca ggcttgggtt gtttcgctac
      361 tcaacaaaga acgattcaaa aatctggcat tcttcatcgg ggaaaatatg gccgatggaa
      421 agggtgaggg tcaggttgca attgtcgaat accgtcaagt cgatggcgaa gaagtgccga
      481 ccctgatgtt ggtgaaagaa gccttaaaca tcgaaaaatg t
//