LOCUS       CO897732                 338 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05h02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:FABH_ASCSU P55776 FATTY ACID-BINDING PROTEIN
            HOMOLOG PRECURSOR, mRNA sequence.
ACCESSION   CO897732
VERSION     CO897732.1  GI:51237522
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 291.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgtccacttt cggaacccaa tgcactcttc gtctctggtg ctcgctctgt tcgcccttct
       61 ggctggcgct ctgaccgccg acgcgggggc cagccaaaat tccggcaaag ttcctcggct
      121 cgtggaccgt ggaccacagc gagggattcg acgagtacct ggaggccaag ggctacggct
      181 ggttcatgcg ccaagtggtg aagctcgcga gcatcaccaa aatattctcg gcgaacgcgg
      241 atgggaccta caactgccag atcgagacga ccaagaagaa cgtggagtgg aaaaatttcc
      301 atctggacaa acaattccag gccgagtacc ttggacca
//