LOCUS       CO897728                 600 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05g09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q20913 Q20913 F57A8.2 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897728
VERSION     CO897728.1  GI:51237518
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 542.
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tccgaattgg tcgtactcgg attcaacgct ggtgacttca actcataccc gtatgctgga
       61 caacagcaac agccgaacga tttctcttat ggtcaatata tcgccccgct ccagaggcaa
      121 ccctatcctc cactacaaaa aaatgttcct gcatcaccac caaattccca gtttccgccg
      181 caaatcgacc ctcaacaatt catgtacgat ccgttgttga acacagctcg ccaattggga
      241 gggcagtttg cagagcaaca aaagcaaaaa ttctcgcagt atttgtccac attcaatttg
      301 aagtattatt ttgctgtgga caccaattat gtcaggagga agttaacaat cattctattt
      361 ccatttatac accgagattg gacgaacaaa tttaccgctg gcgataagcc tgtgaccccg
      421 tgtgaagatg tgaatgctgc tgatttgtac atacccctaa tggccttcat cacatacatt
      481 cttgtctcca gatttgtctt tggcgtccag aaaaggttta cacccgaaaa gttgggcatg
      541 ctgaacacga atgcgttgtt ctacttgccc tttgaaaatg tggtagtctt cgtgaagaaa
//