LOCUS       CO897724                 506 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05g02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO897724
VERSION     CO897724.1  GI:51237514
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgagttgcgc atatcgcatt tcatttgccg cacatcaaag ttctctctct ctctctcagc
       61 gcgcatttgt tgaaaacgaa tagatccgaa ccgagcatgg gcgtgaacac ggagctgctc
      121 caaaaacagg tgcaggcgct aaagctaagc aaagagactg gtggcgacta ctataaagag
      181 ttgttcgccc aacactcgga tgtgtgcgac gcgtacgggg gcattgaccc ggacgcggtg
      241 cccaaatcgg ccaggtacat catgatggcc aacaacgaac tgcaggcctt catgcaattg
      301 ccgaagaact tcaacgagga acgcgactgg cgctcggccc tgtccaactt caaggagcac
      361 tacagcgaca gcgacgtccc gctcaaactc tttaacaaaa caaaggagcc gttcttggcg
      421 ttgctgcaaa agcacgcggg gggaatgagt gctgagcaga aaaagggctg ggaggagctg
      481 ctggacaagg cttacgccga catgaa
//