LOCUS       CO897719                 746 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05f09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO897719
VERSION     CO897719.1  GI:51237509
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 321.
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gttttcttca aatgcgttgc tgctccattc tcactatttt cgctgccatc gtctgtgtgc
       61 tcgccatgct agagttcgcg ccgagtgcgg ccgaccctgt ctccgccagc atgcatgacc
      121 cgcgcttcgg cgccctgatg gaggagcacg acgcagagga cgcaatgtac caccgcatga
      181 agcgctatgg cggatgttgc gattgcaatt gtggatgcgg ttgcgcgtgc tgtggcggct
      241 gctactgcgg gcgcaagaag cgtggggccg aattcctgga gaatgtgctg aaaatccgct
      301 agatgcaggg cgacaactgg attatgcaaa tcaatctagg acttgtatta aattggaaca
      361 tatgtagctt acataattca cttgccaatt gaaaatgtca tttaaaatcc cccgatagtt
      421 aaattaaatg tattttagtt ataaactaag ttttagaaaa taatgaagga ataactatat
      481 tcttttttgt gggaaatatg agagaaataa tttgttttta atgtaagaca gggccagcaa
      541 tgtgaaaatc agggaggggg ttagaggtgt ttttctttat ttcgcttaat cagtatacta
      601 tgtaacacaa aatagataat ttctcatact atattatata ttttatattg cacaataaca
      661 tatacttaat atatatatct acccgcgcta ataataaaaa ttataaataa gagagtaata
      721 ttgacagata aagagaatca aacagt
//