LOCUS       CO897718                 459 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05f07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:IF6_CAEEL O62106 EUKARYOTIC TRANSLATION INITIATION
            FACTOR 6, mRNA sequence.
ACCESSION   CO897718
VERSION     CO897718.1  GI:51237508
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 376.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 attatttaaa atggttgcac ttcgtaccag tttgaggcca gtaacgacgt gggcgtcttt
       61 tgtaaactta ccaatgcgta ttgcttggta ggcattgctg catcggaaaa cttttacagt
      121 gtcgttgaag ccgaacttgc cgacgtgatt ccagtcattc attgttctat ttctggtacg
      181 cgtattgttg gtcggctcac tgttggcaat cggcatggac ttcttgtgcc aagtgcgacc
      241 actgatcaag aattgcagca cattcgcaat agtctgcccg attcggtgca aattcgaaga
      301 attgacgaaa ggctctcagc acttgggaat gttattatat gcaatgacca tgtggctgtg
      361 gttcatgccg atatcancaa aaagacggaa gcagcgcttg ttgaagtact caaagtggaa
      421 gtttttcgac tcaccctatg cgaaaacaca atggttggt
//