LOCUS       CO897717                 382 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05f04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:ARL2_HUMAN P36404 ADP-RIBOSYLATION FACTOR-LIKE
            PROTEIN 2, mRNA sequence.
ACCESSION   CO897717
VERSION     CO897717.1  GI:51237507
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 283.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtctttattg cccaccatcc aaatggtgat gctgaccgtg ttgcggaaag cgaagcgcaa
       61 agagcaagaa atgcgagtgc tcatccttgg gctggacaac tcgggaaaga ccacttttct
      121 gaaacgcatc aacggcgaag acattggcga gacggcgccc accttcggct tcaacatcaa
      181 aacgctggaa tacaaaggct ggaagctgaa ctgctgggat gtgggcgggc agaggagtct
      241 gcgcacctac tggcgcaact acttcgagcg gactgacgcc gtcntctggg tggtggatgc
      301 gtgcgacatc tcgcgcatgg cccacgactg gtgcaaagaa gttcactctc tgatcaacga
      361 ggaatgtctg cccggattga ct
//