LOCUS       CO897712                 744 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05e07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01542 O01542 CODED FOR BY C. ELEGANS CDNA
            YK66C9.5. [1], mRNA sequence.
ACCESSION   CO897712
VERSION     CO897712.1  GI:51237502
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 349.
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcgtctcgca ctactgccgg aggtattggt ttccaggttg tggccaagca ggcgagcaaa
       61 tacagcaacc aggagggaga gatgtgcctg gcctggcatc aagaaaatga gcggcgagaa
      121 tatcagcacc aacggcgacc gtgagaactt ctacaaattg ctaagagacg gcacactact
      181 gttgcaagct ggccaacggg attcaggcgg gctccgtcaa gaagatccaa cagccgactg
      241 gcaccttcgc ctgcatggag aacatccacg ctttcctgtc gttcgcgaag agccagggag
      301 tgcccaccga ggagaccttc cagagcgtgg acttgttcga ggcacgtgac ctctactccg
      361 agtgcatgac cccgttgcca ttgagaccca cctatagtat aacacagaaa gtctttccat
      421 ccatcatcgt tatgattacc gacgaacaaa tgattacttg atttactata aaatgaatat
      481 aaataaaaat tacaatagaa catatcaaca gtaaagctaa actctttaca actataccat
      541 atacctacta atagttctca ttggcccccc tgaatctatt tcataccatc atcaccagca
      601 cttaattctc ctcgatttaa gtcatataaa agattaataa actacccata ccttaagatc
      661 cctcttattt taacgactaa taacgcctat tagcccaaat tctcaccacg ccaaacgcct
      721 ctccatacct actcccctcc cgtt
//