LOCUS       CO897708                 498 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05e03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18405 Q18405 SIMILAR TO MITOCHONDRIAL PRECURSOR
            PROTEINS IMPORT RECEPTORS. [1], mRNA sequence.
ACCESSION   CO897708
VERSION     CO897708.1  GI:51237498
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 528
            Seq primer: SL1 primer
            High quality sequence stop: 492.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gatgtcttcc gactctaaat tgcgcaggag gcagaacagt taaaggtgca gggaaatgaa
       61 tgtttccgac gtcgactata caacaaatct gtctatttct acacacgcgc aattgagtta
      121 gcacctgaaa attcggtgtt gtgggccaac agagcacagt cgtacttgaa tctgcacaaa
      181 ccagaaaagg cattcacgga tgccagtggc gctatccaaa gggattcgaa caatcacaag
      241 gcactgttta gacgagcaca tgcactttgc aaactgggac taagggcgag ggcacgaagc
      301 gatgcactga aatgcgctga gctggaaccg cagaaccctg gagtgagaga gttgttggaa
      361 cagctccagg gtgcatcgga cacgccagcg atagacctca ctgtcttcca gcgtcccgaa
      421 cctcttcaat ccaataaacc gcttgcaaag aagcaaattc atttgtgcaa atgaaaggcg
      481 caatttgaaa aaaaaaaa
//