LOCUS       CO897707                 402 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05d11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O45499 O45499 F39B2.6 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897707
VERSION     CO897707.1  GI:51237497
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tacatagctt aattgaaaca tgaccaagaa acgacgaaac aacgggcgaa acaagaacgg
       61 ccgtggacac gtccagccca ttcgctgcac caactgcgga cgctcctgcc ccaaggacaa
      121 ggcgatcaag aaatttgtgg tccgcaacat tgtggaggcg gccgcagttc gcgacatcac
      181 tgatgcttcg atttacgaac aatacgctct gccgaagctg taccacaagc tgcactactg
      241 tgtttcgtgc gcaattcaca gcaaggtggt gcgcaaccga tcgcgtgagg ctcgtaagga
      301 ccgaaacccg ccgccgcgct tcggacagcg tgcggccgct gaccgcccgc ctcgtcctgg
      361 agcaccagcc gccggaggtc cgggcgctgg agctgggggt cc
//