LOCUS       CO897692                 560 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05b07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q18724 Q18724 C50B8.1 PROTEIN. [1], mRNA sequence.
ACCESSION   CO897692
VERSION     CO897692.1  GI:51237482
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 595
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtttcatat gcgttttgca atgagtcttc tgaatcgtat cactcaaaac atcatacagt
       61 ctggcggcag ccagaattca acaattttcc ttaaacatgt taaatgggta accggtaaat
      121 ctcagcttga gaaccatttt gcgcgttttg gcaaaataaa gagtgtacac atgttctttg
      181 accctgaaac aggattgcac agaggattcg cgtcaataac tttcgagtca ccagaaggag
      241 cttcacaggc catccagaat aagccgcaca taattgatgg agatttggtt aacattcaat
      301 atggccaacc aatgatcaac tacaaacata acaagatgcc catccaacag agaggccata
      361 gccaggtcca gcagagacga cagagcctgc caaatcagag ccaaggaagt actccaccga
      421 aaacttccaa tttagtgccg ccaaacagaa ggacatgatg aggactggag gagatcgact
      481 tgtttgatta gccaatttcc catatctcaa tcatatatgt aaaatgattt tatggttgaa
      541 taaagtggcg tcgtttttaa
//