LOCUS       CO897677                 495 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe05a01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO897677
VERSION     CO897677.1  GI:51237467
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 517
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aactcacctc tcggacgctt tagggcacga gagcgaactt ggacgagtcg agcggcgccc
       61 atctcgactg cacggcgaat ggcgttcatc gcatggaaca gcacccccga ggaggtggat
      121 gcgcccgccg aagcggagga accgaacaac aatggaacaa tgcaaactgg gagcagcgct
      181 gaggaagaag tgaagagcgc cattcggcca acagacggaa caacaacaac agagcaagaa
      241 ggaaacggac gaacaacaaa aacaaagcac agccgcagag tctttctcga cgcattcggc
      301 acccccagca gagtctttca caagccattc attttcggtc tttcatcccc gcaatccacc
      361 atttgttgcc cgcaccccaa aagcccaaaa acagaactac aaacacacac aaacacaatt
      421 gtcgcggtca gcactgtgcc cggaggactg tgaccaaaac atgccctaaa tgtgcgaatg
      481 aataaaacgg attca
//